COLD-PCR is a procedure in the lab, with the advantage of highlighting low-level mutations, but requiring a very precise temperature control system. Its’ principle is based on the notion that mutated DNA has slightly colder denaturization temperatures. By adding an intermediate annealing step at these colder temperatures more of the mutant DNA is replicated compared to the “wildlife” DNA, increasing the likelihood of finding a specific variant.

« Back to Glossary Index
Scroll to Top